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(A) Immunohistochemical analysis of human tonsil shows diffuse staining of <t>kappa</t> light chain (green) within the connective tissue indicating the presence of B cells/plasma cells (red arrows). (B) Immunohistochemical analysis of human gingival tissue from a patient with chronic periodontitis shows prominent staining of kappa light chain (green) within the connective tissue adjacent to the basement membrane (red arrow). In both the tonsil (A) and the gingiva (B), occasional kappa-light-chain–positive cells infiltrate the superficial epithelial layer (yellow arrows). (C) Control staining of human gingival tissue from a patient with chronic periodontitis using <t>FITC-conjugated</t> non-immune <t>IgG.</t> Nuclear staining (blue) by DAPI. E, epithelium; CT, connective tissue. Original magnification 100X. Scale bars, 100 μm.
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(A) Immunohistochemical analysis of human tonsil shows diffuse staining of kappa light chain (green) within the connective tissue indicating the presence of B cells/plasma cells (red arrows). (B) Immunohistochemical analysis of human gingival tissue from a patient with chronic periodontitis shows prominent staining of kappa light chain (green) within the connective tissue adjacent to the basement membrane (red arrow). In both the tonsil (A) and the gingiva (B), occasional kappa-light-chain–positive cells infiltrate the superficial epithelial layer (yellow arrows). (C) Control staining of human gingival tissue from a patient with chronic periodontitis using FITC-conjugated non-immune IgG. Nuclear staining (blue) by DAPI. E, epithelium; CT, connective tissue. Original magnification 100X. Scale bars, 100 μm.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: The B-cell stimulatory cytokines BLyS and APRIL are elevated in human periodontitis and are required for B-cell–dependent bone loss in experimental murine periodontitis 1

doi: 10.4049/jimmunol.1500496

Figure Lengend Snippet: (A) Immunohistochemical analysis of human tonsil shows diffuse staining of kappa light chain (green) within the connective tissue indicating the presence of B cells/plasma cells (red arrows). (B) Immunohistochemical analysis of human gingival tissue from a patient with chronic periodontitis shows prominent staining of kappa light chain (green) within the connective tissue adjacent to the basement membrane (red arrow). In both the tonsil (A) and the gingiva (B), occasional kappa-light-chain–positive cells infiltrate the superficial epithelial layer (yellow arrows). (C) Control staining of human gingival tissue from a patient with chronic periodontitis using FITC-conjugated non-immune IgG. Nuclear staining (blue) by DAPI. E, epithelium; CT, connective tissue. Original magnification 100X. Scale bars, 100 μm.

Article Snippet: To this end, the following Abs were used: goat anti-human APRIL polyclonal IgG (Abcam), goat anti-human BLyS polyclonal IgG (R&D Systems), and FITC-conjugated rabbit anti-human Ig kappa light chain polyclonal IgG (DakoCytomation) that recognizes B cells and Ig-secreting plasma cells ( 30 ).

Techniques: Immunohistochemical staining, Staining